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Antibodies bound to the blocking peptide no more bind for the epitope on the focus on protein. By comparing the staining with the blocked antibody compared to the antibody by itself, you can see which staining is specific.

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This tutorial outlines additional possible optimization of cellular and immunoassay parameters to make sure the very best final results are...

This tutorial outlines even more feasible optimization of mobile and immunoassay parameters to make sure the best possible benefits are...

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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...

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Blocking peptides are peptides that bind specially to your focus on antibody and block antibody binding. These peptide commonly is made up of the epitope identified through the antibody.

Goal Info Cyclin E belongs for the very conserved cyclin family members, whose associates are characterised by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E types a posh with and features being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development by means of S phase.

Overexpression of the gene continues to be observed in lots of tumors, which results in chromosome instability, and so might add to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in promoting cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually explained. Two more splice variants had been described but detailed nucleotide sequence data is not really but readily available.

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